Research Associate - IITA

Key Responsibilities

The position focuses on performing genomic DNA extractions, PCR, Southern blotting, qRT-PCR, sequencing, mutation detection, and maintaining bacterial cultures. The successful candidate will work closely with a multidisciplinary team of scientists and contribute to ongoing research in plant genome editing and trait enhancement.

• Perform genomic DNA extraction from various transgenic and gene edited events of banana, yam or any other crops for molecular analysis.
• Conduct PCR (Polymerase Chain Reaction) and qRT-PCR (quantitative Real-Time PCR) to assess gene expression and mutation verification.
• Perform mutation detection assays, including T7 endonuclease and band-shift analysis.
• Carry out Southern blot analysis for gene copy number determination and transgene integration.
• Sequence analysis for detection and validation of gene edits using techniques such as Sanger or next-generation sequencing.
• Maintain and propagate bacterial cultures for plasmid DNA preparations.
• Prepare media and assist in sub-culturing the plants. Assist in the maintenance of gene-edited plant lines in the lab and greenhouse
• Ensure accurate data collection, analysis, and record-keeping for experimental results – record and analyse data
• Assist in preparing reports, presentations, and manuscripts related to the research findings.
• Collaborate with other team members and provide technical support in molecular techniques.
• Perform any other duty assigned

Requirements

• Bachelor’s degree in molecular biology, Biotechnology, Genetics, or a related field with 1 year of experience or Diploma in relevant field with 3 yrs experience
• Relevant professional certification
• Hands-on experience with molecular techniques such as DNA extraction, PCR, qRT-PCR, and sequencing.
• Familiarity with Southern blotting and mutation detection assays is a plus.
• Ability to maintain detailed lab records and follow protocols with precision.
• Strong organizational and time management skills.
• Excellent interpersonal and communication skills for working in a collaborative research environment.